Substitution of Egg Yolk with Arabic Gum in Extenders Used for Ram Semen Cold Storage or Cryopreservation

The current research was designed to compare the effects of substitution egg yolk (EY) in ram extender with Arabic gum (AG) different concentration cooled or frozen semen collected by artificial vagina (AV) electro-ejaculator (EE) Noemi rams. Semen 2 times per week for 6 weeks from rams EE method and after a rest period weeks, samples were AV method. Tris either contained 15% EY (Control) 3%, 5% 7% AG (AG3, AG5 AG7, respectively) used as extenders. Spermatozoa evaluated cold storage (4 °C) freezing/thawing liquid nitrogen sperm motility parameters: total motile spermatozoa (% TMS), rapid progressively PRS), curvilinear velocity (VCL) μm/s, rectilinear (VSL) average path (VAP) straightness index STR), linearity coefficient LIN), morphological defects acrosome membrane integrity. Results showed that AV-collected diluted more efficient than EE. values VCL, VSL VAP higher AG3 extenders compared After storage, gave better (P<0.01) results LIN STR Significant differences morphology noted regardless used, however, supplementation enhance (P<0.05). Post-freezing, containing had TMS, PRS, others. Sperm AG7 group. Plasma integrity significantly (P<0.05) AV-group control other groups. AV-semen groups greater EE-semen It can be concluded that, is good candidate replace when added at 3 5%. Although collection electro-ejaculator. However, could electro-ejaculator-collected characteristics